Figure 2 | Cell Death & Disease

Figure 2

From: Development of a human three-dimensional organotypic skin-melanoma spheroid model for in vitro drug testing

Figure 2

Generation of the organotypic melanoma-spheroid skin model. (a) 250 451-LU melanoma cells per 25 μl drop RPMI medium were placed on the lid of a non-adhesive PBS containing petri dish and incubated for 15 days deriving melanoma spheroids with a size of about 500 μm in diameter. Live/death staining was performed by incubation with 5 μg fluorescein diacetate (FDA) and 45 g propidium iodide (PI)/ml PBS for 20 min at RT in the dark. Living cells stain FDA positive (green) and dead cells stain PI positive (red). (b) Ten 451-LU spheroids were collected, carefully mixed with primary fibroblasts and added to collagen I to from the dermal compartment of the full thickness skin equivalent

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