Figure 3

KPNA2 is essential for EOC cell tumorigenicity in vitro and in vivo. Representative micrographs (left panel) and quantifications (right panel) of soft agar colony formation assays for the following cell lines, relative to the control: (a and b) EFO-21-vector, EFO-21-KPNA2, EFO-21-si-scramble, EFO-21-siKPNA2 #1, EFO-21-siKPNA2 #2; and (c and d) SK-OV3-vector, SK-OV3-KPNA2, SK-OV3-si-vector, SK-OV3-siKPNA2 #1, SK-OV3-siKPNA2 #2. Each bar represents the mean (± S.D.) of three independent experiments. Xenograft model in nude mice. (e) EFO-21-vector, EFO-21/KPNA2 (KPNA2), EFO-21-si-scramble, and EFO-21/siKPNA2 #1 (siKPNA2) cells were injected subcutaneously into the left and right flanks of the mice. (f) Representative pictures of tumor growth 30 days after inoculation. (g) Tumor volumes were measured on the indicated days. All data are shown as mean±S.D. (h) Western blotting analysis of KPNA2 expression in EFO-21-vector, EFO-21/KPNA2 (KPNA2), EFO-21-si-scramble, and EFO-21/siKPNA2 #1 (siKPNA2) generated tumors 30 days after injection. (i) Immunohistochemical analysis of EFO-21-vector, EFO-21/KPNA2 (KPNA2), EFO-21-si-scramble, and EFO-21/siKPNA2 #1 (siKPNA2) generated tumors 30 days after injection. Sections obtained from tumors were incubated with anti-ki67 antibody. Representative fields are shown ( × 200 magnification) *P<0.05; **P<0.01