Figure 6 | Cell Death & Disease

Figure 6

From: KPNA2 promotes cell proliferation and tumorigenicity in epithelial ovarian carcinoma through upregulation of c-Myc and downregulation of FOXO3a

Figure 6

Downregulation of KPNA2 decreases transcriptional activity of c-Myc and the activity of Akt, and increases the activity of FOXO3a. (a and b) c-Myc reporter activity in EOC cells transduced with vector KPNA2; si-scramble; siKPNA2 #1; and siKPNA2 #2, relative to the control. (c and d) Western blotting analysis of phosphorylated Akt (p-Akt), total Akt, phosphorylated FOXO3a (p- FOXO3a), total FOXO3a, and c-Myc proteins in the indicated EOC cells. (e and f) Expression levels were quantitated using ImageJ software (Wayne Rashband; bottom); β-actin was used as a loading control. Error bars represent the S.D. from three independent experiments. *P<0.05 compared with the control

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