Figure 1 | Cell Death & Disease

Figure 1

From: Thrombocytopenia induced by the histone deacetylase inhibitor abexinostat involves p53-dependent and -independent mechanisms

Figure 1

The pan HDACi abexinostat (A) decreases hematopoietic progenitors growth. Representative experiments are shown to illustrate the number of progenitors generated in a semi-solid assay in the presence of indicated doses of abexinostat, n=3. In each experiment, CD34+ cells were seeded in triplicate. Results: mean±S.E.M., ***P<0.0001; **P<0.004; *P<0.05. (a and b) Number of BFU-E, CFU-GM CFU-GEMM and total colonies (total CFU) generated by plating 500 CD34+ cells from leukapheresis or bone marrow samples in methylcellulose assays in the presence of SCF+IL-3+EPO+G-CSF+ IL-6 as described in Materials and Methods. (a) Leukapheresis samples; (b) bone marrow exposed all along the culture or only 24 h to abexinostat. (c) Number of CFU-MK colonies generated by plating 1000 CD34+ cells in a fibrin clot assay in the presence of TPO+SCF+IL-6. (d and e) Number of CFU-MK colonies generated by plating CD34+ cells from leukapheresis (d) exposed 24 h to the indicated concentration of abexinostat, then washed before plating 500 CD34+ cells or from bone marrow samples exposed all along the culture or only 24 h to abexinostat (e)

Back to article page