Figure 2

Abexinostat induces a decrease of cellular growth, an increase in apoptosis and a decrease of ploidy. (a) CD34⁺ cells were seeded in liquid culture in the presence of TPO±increasing dose of abexinostat, viable cells were counted using trypan blue exclusion at day 6 and results are the mean±S.E.M. (representative experiments of n=3;*P<0.05; **P<0.004; ***P<0.002). (b and c) CD34⁺ cells were seeded with TPO±increasing dose of abexinostat for 48 h then stained with allophycocyanin-Annexin V-propidium iodide. The percentage of apoptotic cells was determined by flow cytometry. (b) Mean of three independent experiments; (c) representative experiments. *P<0.04; **P<0.001, ***P<0.0001. (d and e) Mean ploidy value of MKs at day 10 of culture with indicated doses of abexinostat (d) mean of three independent experiments: control N: 3.82; 10 nM: 3.66; 50 nM: 2.59; 100 nM: 2.35. *P<0.03; (e) representative ploidy experiment. (f) Mean ploidy value at indicated times, with 100 nM abexinostat added at day 1 or 8. Control at day 8N: 3.80; control at day 10N: 4.7; day 10 analysis for 100 nM abexinostat added either at day 1 (N: 2.51) or at day 8 (N: 4.03)