Figure 6 | Cell Death & Disease

Figure 6

From: Thrombocytopenia induced by the histone deacetylase inhibitor abexinostat involves p53-dependent and -independent mechanisms

Figure 6

Activation of p53 in abexinostat-treated MK. CD41+ cells were sorted at day 7 of culture and seeded in serum-free medium with TPO with or without indicated doses of abexinostat for 48 h. (a) Immunoblot analysis of the phosphorylation status of ATM and γH2AX. (b) Immunoblot analysis of caspase 3 in sorted MK treated with or without abexinostat (50 and 100 nM). (c) Immunoblot analysis of the phosphorylated p53 on serine residue 15, BAX and BCL-xL. HSC70 used as loading charge. (d) qRT-PCR studies of p53 target genes. Data are mean±S.E.M., n=3, each experiments performed in triplicate, ***P<0.0007

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