Figure 7

p53 knockdown rescues MK apoptosis, but not proplatelet formation. (a) Immunoblot analysis of p53 performed on sorted MK transduced with shSCR or shp53. (b) Untreated or treated MKs were stained with Annexin V and analyzed by flow cytometry. Data represent the mean±S.E. of the % of Annexin V⁺ cells, n=2. In these two independent experiments, results show that the shp53 markedly reduced the apoptosis induced by abexinostat in comparison to the SCR. (c and d) Proplatelet formation derived from shp53-treated and non-treated MK. shp53 increased proplatelet formation by MKs in control cultures (P<0.0007), and in 50 nM abexinostat cultures (**P<0.002) (d). At 100 nM abexinostat, MKs kept their round shapes and remained alive but did not form proplatelets. ***P<0.0007