Figure 6 | Cell Death & Disease

Figure 6

From: Differential effect of long-term drug selection with doxorubicin and vorinostat on neuroblastoma cells with cancer stem cell characteristics

Figure 6

The side population of SK-N-Be(2)C (a) and SK-N-SH (b) cell lines was analyzed. A representative chart of a total of three experiments was shown. Left: Hoechst 33342 dye treatment only. Middle: 50 μM verapamil treatment. Right: 100 μM verapamil treatment. All cells were incubated with Hoechst 3342 dye, excited at 357 nm. Its fluorescence was analyzed at two wavelengths (blue: 670–700 nm and violet: 450–500 nm), both in linear mode. Incubation with propidium iodide (1 μg/ml) was performed to exclude dead cells immediately before analysis. Singlets were gated for side-population analysis. Side-population cells are shown by the outlined area

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