Figure 2

Qualitative RT-PCR for P2X receptors. ASCs were found to express mRNAs for P2X3 and P2X4 receptors. P2X4 and P2X7 receptors were upregulated in dASC. However, P2X6 transcripts were not detected in ASC despite increasing the amount of the starting RNA template. aSC, nSC total RNA were used as positive controls. β-actin was used as a loading control and a reaction omitting the template was used as a negative control