Table 1 Specific primers used for RT-PCR studies

From: Differentiation of adipose-derived stem cells into Schwann cell phenotype induces expression of P2X receptors that control cell death

Gene

AN GenBank

Primer sequence (5′–3′)

AT (°C)

PL (bp)

P2X 1

X80447

F: GAAGTGTGATCTGGACTGGCACGT

58

452

  

R: GCGTCAAGTCCGGATCTCGACTAA

  

P2X 2

U14414

F: GAATCAGAGTGCAACCCCAA

61

357

  

R: TCACAGGCCATCTACTTGAG

  

P2X 3

X90651

F: TGGCGTTCTGGGTATTAAGATCGG

58

440

  

R: CAGTGGCCTGGTCACTGGCGA

  

P2X 4

X87763

F: GAGGCATCATGGGTATCCAGATCAAG

58

447

  

R: GAGCGGGGTGGAAATGTAACTTTAG

  

P2X 5

X92069

F: GCCGAAAGCTTCACCATTTCCATAA

58

418

  

R: CCTACGGCATCCGCTTTGATGTGATAG

  

P2X 6

X92070

F: AAAGACTGGTCAGTGTGTGGCGTTC

64

520

  

R: TGCCTGCCCAGTGACAAGAATGTCAA

  

P2X 7

X95882

F: GTGCCATTCTGACCAGGGTTGTATAAA

58

354

  

R: GCCACCTCTGTAAAGTTCTCTCCGATT

  

β-actin

NM031144

F: CACCACAGCTGAGAGGGAAATCGTGCGTGA

R: ATTTGCGGTGCACGATGGAGGGGCCGGACT

60

510

  1. Abbreviations: AN GenBank, accession number; AT, annealing temperature; F, forward; PL, product length; R, reverse.
  2. Sequences from Shibuya et al61
Back to article page