Figure 7 | Cell Death & Disease

Figure 7

From: A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells

Figure 7

Bcl family members regulate compound K-induced autophagy. HCT-116 cells were treated with compound K (20 μg/ml) for the indicated times. (a) Bcl-2 and phospho-Bcl-2 levels were assessed by western blot analysis. (b) Cell lysates were subjected to immune-precipitation with an antibody against Atg6, and the immune-precipitates were subjected to western blot analysis with an antibody against Bcl-2. Compound K-provoked apoptosis was assessed by (c) flow cytometry and (d) western blot analysis in 3-MA pre-treated cells. *Significantly different from control (P<0.05) and #significantly different from compound K-treated cells (P<0.05)

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