Figure 1
From: Inhibition of H3K9 methyltransferases G9a/GLP prevents ototoxicity and ongoing hair cell death
The global H3K9me2 levels increased rapidly upon hair cell damage. (a) H3K9me2 immunofluorescence was detected as punctuate staining in normal hair cells cultured as organ culture in serum-free medium. (b and c) The relative fluorescent intensity of H3K9me2 increased significantly after the addition of 1 mM neomycin for 15 min or 3 h when compared with the untreated group. (d) The H3K9me2 level increased after all of the following treatments: 100 μM cisplatin treatment for 3 h, 50 μM copper (CU) treatment for 3 h, ultraviolet (UV) irradiation for 15 min, or 1 mM neomycin treatment for 3 h, as confirmed by western blot analysis (n=12 per group in each trial) and quantified by the grey-degree numerical value in the form of mean±S.E. values obtained from three replicates. Shown in the images is the middle turn segment. The red spots represent the H3K9me2 signal, the green fluorescence shows staining of the hair cells and the nuclei are labelled with 4,6-diamidino-2-phenylindole (DAPI). Bar=10 μm. **P<0.01. DDP; cisplatin; CON, control group
