Figure 2
From: Inhibition of H3K9 methyltransferases G9a/GLP prevents ototoxicity and ongoing hair cell death
The H3K9me2 level decreased with BIX01294 treatment in hair cells. (a) H3K9me2 level is relatively uniform across the hair cell epithelium within the organs of Corti cultured in serum-free medium. Bar=20 μm. (b) H3K9me2 significantly decreased with the addition of 2 μM BIX01294 for 24 h. Bar=20 μm. (c) Hair cell loss was observed in the presence of 10 μM BIX01294 for 24 h in the basal segment. Bar=20 μm. (d) The dose-dependent inhibition of H3K9me2 in cultured organ of Corti was confirmed by western blot analysis (n=12 per group) and quantified by the grey-degree numerical value from three replicates. Analysis of variance and SNK-q test, **P<0.01. DAPI, 4,6-diamidino-2-phenylindole. CON, control group
