Figure 3
From: Inhibition of H3K9 methyltransferases G9a/GLP prevents ototoxicity and ongoing hair cell death
Assessment of hair cell survival and apoptosis upon neomycin treatment with or without BIX01294. (a–l) Organs of Corti were cultured in serum-free medium. They were either treated with neomycin alone (Neo), neomycin with BIX01294 pre-treatment (BIX-Neo), neomycin and BIX01294 co-treatment (co-treatment), or neomycin with BIX01294 post treatment (Neo-BIX). Shown here are the confocal images of the apical, middle, and basal turns from these four groups, respectively. The hair cells were labelled with Math1-GFP (Math1-green fluorescent protein; green), and the nuclei were DAPI (4,6-diamidino-2-phenylindole) stained (blue). Arrows in f, h, i, k, and l indicate the apoptotic bodies with condensed or fragmented nuclei. Bar=5 μm. (m) Quantitative analysis of hair cell protection by BIX01294. The samples were divided into six segments of equal length, which was called segment 1, 2, 3, 4, 5, and 6 from apex to base. We counted the number of hair cells in each visual field with the same length of each segment for each treatment group, data are expressed as mean±S.E. (the length is 360 μm, n=8 per group, *P<0.05, **P<0.01.). (n) Quantification of the apoptotic bodies (the length is 360 μm, n=8 per group, *P<0.05, **P<0.01)
