Figure 7
From: Inhibition of H3K9 methyltransferases G9a/GLP prevents ototoxicity and ongoing hair cell death
BIX01294 treatment affects mitochondrial apoptosis pathway. (a and b) Mitochondrial membrane permeability (MMP) staining of the organs of Corti in neomycin-induced hair cell damage model with or without BIX01294. The red fluorescence shows the live-cell imaging of the MMP tagged with TMRM, the green fluorescence represents the hair cells labelled with Math1-GFP (Math1-green fluorescent protein), and blue represents DAPI (4,6-diamidino-2-phenylindole) staining of the nuclei. Shown here are the confocal images of the middle segments of each group. The TMRM staining in the hair cells decreased significantly with the neomycin-only treatment, whereas it only slightly decreased in the presence of BIX01294. Bar=20 μm. (c) The relative fluorescence intensity of TMRM in the control group and BIX01294 pre-conditioned group (n=3 per group). (d and e) The level of cleaved caspase-3 decreased in the presence of 2 μM BIX01294 when compared with the neomycin-only-treated organ of Corti. Shown here are the confocal images of the middle segments of each group. Bar=20 μm. (f) The average level of cleaved caspase-3 was measured using western blot (n=12 per group in each trial) and plotted in the form of mean±S.E. values obtained from three replicates. β-actin was used as the loading control
