Figure 8
From: Inhibition of H3K9 methyltransferases G9a/GLP prevents ototoxicity and ongoing hair cell death
Inhibition of G9a/GLP protects auditory hair cell death in the organ of Corti in vivo. (a–f) SEM examination of the stereocilia in the organ of Corti was performed from the apical turn to the basal turn in the neomycin-exposed animal with or without the BIX01294 pre-treatment. There is less severe hair cells loss in the BIX01294 pre-treatment group as compared with the untreated group. Bar=5 μm. (g–l) Similar results were obtained using confocal microscopy of Myosin7a (red) and nuclear (blue) staining. Bar=10 μm. (m) Quantification of the surviving hair cells at the apical, middle, and basal turns of the cochlea of each group. The length used for the statistical analysis was 100 μm, n=9 in each group. Data are expressed as mean±S.E. (Student’s t-test, *P<0.05, **P<0.01). (n) A sketch was drawn to show the ‘transitional’ zone for the different conditions: BIX-Neo (#) versus Neo (*). (o) The mean ABR threshold was compared between left and right ears, that is, the ears with or without BIX01294 pre-treatment, n=10 in each group. Data are expressed as mean±S.E. (Student’s t-test, *P<0.05, **P<0.01)
