Figure 2 | Cell Death & Disease

Figure 2

From: Rad54 is required for the normal development of male and female germ cells and contributes to the maintainance of their genome integrity after genotoxic stress

Figure 2

Rad54 is required for normal germ cell development. (a) Immunohistochemical staining of DDX4 (purple) and cleaved-caspase-3 (brown) in P4 testes cross sections from WT and Rad54 KO mice. (b) Total germ cell count (DDX4-positive cells) analysis in both type of mice at different ages. (c) Percentage of apoptotic cells (cleaved caspase-3 positive cells) in E13.5 and P4 testes from WT and Rad54 KO mice. (d) Flow cytometric analysis of the germ cell and somatic cell absolute numbers in E14.5 WT and KO mice, using beads as internal control. (e) Testis cross section from E11.5 WT and KO stained for DDX4 (purple) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (brown). (f) PGC number in E11.5 fetuses counted on all sections of total embryo. At least five animals per group were analyzed except for E11.5 (n=3–4) embryos. Data are presented as mean±S.E.M. Statistically significant differences between WT and KO are indicated by asterisks (*P<0.05; **P<0.01)

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