Figure 6 | Cell Death & Disease

Figure 6

From: Rad54 is required for the normal development of male and female germ cells and contributes to the maintainance of their genome integrity after genotoxic stress

Figure 6

Follow-up of spermatogonial germ cell subpopulations after P3 irradiation. (a) Immunohistological analysis of the germ cell numbers (DDX4-positive cells) in testes from WT and KO mice 24 h after irradiation at P3 (2 Gy). Data are presented as the percentage of the non-irradiated control (mean±S.E.M; n=4). (b) Testicular GDNF production is induced within the first 24 h after irradiation of KO P3 mice only (n=5). (c) Flow cytometric analysis of the spermatogonial subpopulation absolute numbers at different ages. Four to six animals per group were analyzed. (d) Immunohistochemical analysis of the Plzfhigh-spermatogonial subpopulation per seminiferous tubule cross section (PLZF: green; BrdU: red; DAPI: blue) (n=3). Statistically significant differences between irradiated and non-irradiated gonads are indicated by asterisks (*P<0.05; **P<0.01; ***P<0.001)

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