Figure 1 | Cell Death & Disease

Figure 1

From: Retinoblastoma protein regulates the crosstalk between autophagy and apoptosis, and favors glioblastoma resistance to etoposide

Figure 1

RB knockdown in GBM cells and GSCs. (a) Representative western Blotting image of three independent experiments, comparing the levels of RB protein between non-silenced (siRNA-Neg) and silenced groups (siRNA-RB) of U87 and GBM95 cell lines. α-Tubulin was used as loading control. (b) Histogram comparing RB levels between silenced (siRNA-RB) and non-silenced groups (siRNA-Neg) of GBM cell lines U87 and GBM95. Data are represented as mean and S.E.M. of three independent experiments. (c) Representative western blotting image of three independent experiments, comparing the levels of RB protein between non-silenced (siRNA-Neg) and silenced groups (siRNA-RB) of GSCs OB1. α-Tubulin was used as loading control. (d) Histogram comparing RB levels in GSCs OB1 between silenced (siRNA-RB) and non- silenced groups (siRNA-Neg). Data are represented as mean and S.E.M. of three independent experiments. (e) VP-16 dose–response curves of GBM cell lines U87 and GBM95, and GSCs OB1. Graphic shows the % of survival as measured by MTT method by the indicated dose treatment of VP-16 for 24 h. Data are represented as mean and S.E.M. of three independent experiments

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