Figure 3 | Cell Death & Disease

Figure 3

From: Retinoblastoma protein regulates the crosstalk between autophagy and apoptosis, and favors glioblastoma resistance to etoposide

Figure 3

RB knockdown increased apoptosis induced by VP-16 in different GBM cell lines. (a) Representative images of three independent experiments of TUNEL assay (red) using A172, T98G and U373 cells after 24 h of 1 μM VP-16 treatment; experimental groups as indicated. Nuclei were stained with DAPI. Arrows indicate TUNEL-positive cells. Scale bar=10 μm. (b) Percentage of TUNEL-positive A172 (checkered bars), T98G (horizontal striped bars) and U373 (gray bars) cells 24 h after 1 μM VP-16 treatment; experimental groups as indicated. **P<0.01 and ***P<0.005 by ANOVA and Tukey’s post test. Data are represented as mean and S.E.M. of three independent experiments. (c) Representative western Blotting image of three independent experiments, comparing the levels of RB protein between non-silenced (siRNA-Neg) and silenced groups (siRNA-RB) of A172, T98G and U373 cell lines. α-Tubulin was used as loading control

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