Figure 4 | Cell Death & Disease

Figure 4

From: Retinoblastoma protein regulates the crosstalk between autophagy and apoptosis, and favors glioblastoma resistance to etoposide

Figure 4

RB knockdown increased DNA double-strand breaks induced by VP-16. (a) Representative images of three independent experiments of immunofluorescence staining with anti-γH2AX antibody (red) after 1 h of VP-16 treatment in U87 cells; experimental groups as indicated. Nuclei were stained with DAPI. Scale bar=10 μm. (b) Histogram shows fold changes of red fluorescence intensity (mean red fluorescence/total cell number) over siRNA-Neg control group; after 1 h of VP-16 treatment of U87 (black bars) and GBM95 (white bars) cells; experimental groups as indicated. ***P<0.005 by ANOVA and Tukey’s post test. Data are represented as mean and S.E.M. of three independent experiments. (c) Representative γ-H2AX immunoblot of U87 and GBM95 cells 1 h post-VP-16 treatment; experimental groups as indicated. α-Tubulin was used as loading control

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