Figure 5
WFA promotes bone regeneration in the drill-hole site in sham-operated (ovary intact) and OVx mice. (a) Representative confocal images (magnification= × 100) of calcein labeling shown in the drill-hole site of various groups 0, 11 and 21 days after injury without and with WFA treatment(5 and 10 mg/kg/day). (b) Data show the quantification of the mean intensity of calcein labeling. Values represent mean±S.E. **P<0.01, ***P<0.001 compared with Sham vehicle. #P<0.05, ##P<0.01, ###P<0.001 compared with OVx vehicle group. Inter-dose comparison shows that cP<0.05 when 10 mg/kg/day dose was compared with 5 mg/kg/day dose. *P<0.05 when OVx+V compared with Sham+V. (c) Representative μCT images from the center of the bony hole (defect region) on days 0, 11 and 21 in the sham and OVx groups. (d and e) Graph represents μCT analysis in the defect and intra-medulla regions of sham and sham+ WFA-treatment group. Data show BV/TV (%) at 5 and 10 mg/kg/day dose in both the regions.Values represent mean±S.E. **P<0.01, ***P<0.001 compared with Sham vehicle. ##P<0.01compared with OVx vehicle. Inter-dose comparison shows aP<0.05 when 10 mg/kg/day dose is compared with 5 mg/kg/day dose in sham group on day 11. (f and g) Graph represents μCT analysis in the defect and intra-medulla regions of OVx and OVx+ WFA treatment groups. Values represent Mean±S.E. *P<0.05, ***P<.001 compared with Sham vehicle. #P<0.05 and ##P<0.01 compared with OVx vehicle. Inter-dose comparison shows aP<0.05 when 10 mg/kg/day dose is compared with 5 mg/kg/day dose in sham group on day 11. (h–j) Effect of WFA on osteogenic genes after drill-hole injury. mRNA profile of RunX2, BMP2 and OCN extracted from callus of drill site of bone. Values represent mean±S.E. of three independent experiments n=3. **P<0.01, ***P<0.001 compared with Sham vehicle. #P<0.05, ##P<0.01, ###P<0.001 compared with OVx vehicle. Inter-dose comparison shows aP<0.05 when 10 mg/kg/day dose is compared with 5 mg/kg/day dose in sham group on day 11. bP<0.05 when 10 mg/kg/day dose is compared with 5 mg/kg/day in sham group on day 21. cP<0.05 when 10 mg/kg/daydose iseffective than 5 mg/kg/day in OVx group on day 11. dP<0.05 when 10 mg/kg/day dose is effective than 5 mg/kg/day in OVx group at day 21for RunX2 gene
