Figure 6 | Cell Death & Disease

Figure 6

From: Hyperactivation of retina by light in mice leads to photoreceptor cell death mediated by VEGF and retinal pigment epithelium permeability

Figure 6

Blocking VEGF in the LD model preserved the rod and cone outer segment morphology. Peripherin–RDS expression, normally localized in rod and cone OS of control unexposed to light (a), is completely abolished 10 days after light exposure (b). Peripherin–RDS expression is also absent in the sham-treated retina (c). Although Peripherin–RDS staining is observed in both LV-GFP- (d) and LV-V65 (e)-treated retinas, the OS in the LV-GFP-treated group appeared shorter and disorganized compared with those of LV-V65-treated group. In the LV-V65-treated group, the OS remains well organized and has an appropriate length. Similar result was obtained for the cone OS marker SWL–opsin analyzed 10 days after LD. SWL–opsin staining, normally found in the cone OS of control unexposed to light (f and f′), is completely abolished after light exposure (g and g′). Similarly, the expression of SWL–opsin is absent in sham-treated retina (h and h′). SWL–opsin expression is maintained in LV-GFP- (i and i′) and LV-V65(j and j′)-treated groups; however, in addition to a weaker expression in the LV-GFP-treated group, the protein was partly mislocalized to the axon and cone pedicle (i′) (n=8 per group – magnification × 400, scale bar: 100 μm – red: Peripherin/SWL–opsin and blue: Dapi)

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