Figure 3 | Cell Death & Disease

Figure 3

From: Neuronal STAT3 activation is essential for CNTF- and inflammatory stimulation-induced CNS axon regeneration

Figure 3

STAT3 depletion impairs IS-induced neuroprotection. (a) Immunohistochemical staining of surviving RGCs in retinal flat-mounts 14 days after optic nerve crush (onc) or onc+inflammatory stimulation (onc/is) using an antibody against βIII tubulin. Scale bar: 50 μm. Animals were intravitreally injected with either AAV2-GFP (gfp) or AAV2-Cre (cre) 2 weeks before surgery. (b) Quantification of surviving RGCs per mm2 in retinal flat-mounts of animals treated as described in a. Conditional STAT3 knockdown partially reduced the neuroprotective effect of IS. Treatment effects: n.s., non-significant; *P<0.05; ***P<0.001. Values represent the mean of at least five retinae per treatment

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