Figure 6
From: Characterization of oxygen radical formation mechanism at early cardiac ischemia

Localization of O2•– formation during the onset of ischemia on the heart surface using confocal microscopy. (a) heart baseline fluorescence before ischemia. (b) O2•– generation in the HE-loaded heart within 3 min during ischemia. (c) O2•– generation in hearts treated with dual-fluorescence probes (HE and fluorescein lectin I-isolectin B4 (FLI-IB4)), showing that O2•– production is within the cardiomyocytes, not in the endothelial vessels