Figure 5 | Cell Death & Disease

Figure 5

From: Neoalbaconol induces energy depletion and multiple cell death in cancer cells by targeting PDK1-PI3-K/Akt signaling pathway

Figure 5

NA-induced dysfunction of glucose metabolism results in cell death. (a) Viability of C666-1 or HK1 cells treated with DMSO, NA(40 μM), NA/Nec-1(40 μM), NA/zVAD-fmk(20 μM), NA/Nec-1/zVAD-fmk, NA/3-MA(5 mM), Nec-1, zVAD-fmk, Nec-1/zVAD-fmk or 3-MA was analyzed by MTS assay. Data are shown as mean+S.D. of three experiments. *P<0.05. **P<0.001. (b) The effect of the autophagy inhibitor 3-MA(5 mM) or JNKs inhibitor SP600125(50 μM), apoptosis inhibitor zVAD-fmk(20 μM) and necroptosis inhibitor Nec-1 on the level of JNKs, phosphorylated JNKs, PARP-1 and LC3 was analyzed in C666-1 cells treated or not treated with NA. β-Actin served as a loading control. (c) The C666-1 cells were pretreated with SP600125 (50 μM) for 1 h and then treated with or without NA (40 μM) for an additional 24 h. The cell viability was analyzed by MTS. Data are shown as mean+S.D. of three experiments. *P<0.05

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