Figure 1 | Cell Death & Disease

Figure 1

From: Lithium chloride attenuates cell death in oculopharyngeal muscular dystrophy by perturbing Wnt/β-catenin pathway

Figure 1

Choosing the best concentration of LiCl on the C2C12 cell line. LiCl (GSK inhibitor) at 2.5 mM maintains growth, size, and proliferation of C2C12 muscle cells. A dose-response experiment on non-transfected C2C12 cells shows that with a low dose of 2.5 mM LiCl, the cells maintain their growth and survival similarly to untreated cells. Cells were monitored under a live-stage Leica DMI 6000 microscope for morphology and viability. Phase images were captured at the indicated days after LiCl treatment. Higher concentrations of LiCl at 5 and 15 mM (top two rows) were toxic and caused the cells to die, while a concentration of 2.5 mM LiCl maintained the cells healthy and in good shape for 6 days. Therefore, 2.5 mM LiCl was the chosen concentration throughout the study

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