Figure 6 | Cell Death & Disease

Figure 6

From: Lithium chloride attenuates cell death in oculopharyngeal muscular dystrophy by perturbing Wnt/β-catenin pathway

Figure 6

LiCl leads to a significant increase of the β-catenin level in the OPMD C2C12 cell model. (a and b) LiCl leads to a significant increase of β-catenin in the OPMD C2C12 cell model while leaving the GFP level not affected. A western blot showing the effect of LiCl treatment on the β-catenin level at 48 h post transfection. C2C12 myoblast cells were transiently transfected with different PABPN1constructs as well as GFP, and non-transfected cells, and treated or not with two different doses of LiCl as indicated. Actin antibody was used to confirm equal loading. (c and d) LiCl treatment results in re-distribution of β-catenin from a cytoplasmic to a nuclear compartment. Confocal images of immunocytochemistry showing the subcellular distribution of β-catenin. A clear accumulation of β-catenin in the nucleus was observed in the C2C12-transfected cells with GFP-wtPABPN1-10Ala (c) and GFP-expPABPN1-17Ala (d) after LiCl treatment. GFP-PABPN1 constructs (green), β-catenin (red)

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