Figure 2
Suppressed osteoblastogenesis and increased adipogenesis of Dex-induced osteoporotic BMSCs. (a) Proliferation of BMSCs from both Dex- and Saline-treated mice measured by MTT assay. (b) Apoptosis of BMSCs cultured in growth medium were detected by TUNEL staining. (c) Relative expressions of osteoblast and adipocyte markers in BMSCs from Dex- or saline-treated mice under the induction of BMP2 to indicated times were quantified by qRT-PCR. (d) Relative expressions of adipocyte genes in BMSCs from Dex- or saline-treated mice were quantified by qRT-PCR in transdifferentiation assay. (e) Oil red O staining of the transdifferentiated adipocytes from indicated cells showed higher adipocyte conversion potential of osteoporotic BMSCs. N=3. Respective cells cultured in the growth medium were used as control. (f) The expressions of osteoblast and adipocyte markers in C3H10T1/2 cells treated as indicated were quantified by qRT-PCR. (g) Relative expressions of adipocyte genes in transdifferentiated C3H10T1/2 cells that were treated with different doses of Dex. (h) Oil red O staining shows the transdifferentiated adipocytes from cells treated by 10–6 M Dex. Cells cultured in the growth medium were used as control. N=3. All mRNA expression levels were normalized to beta-actin. Data are presented as mean±S.D. of three independent experiments. *P<0.05, **P<0.01, n.s. P>0.05, N=3
