Figure 5 | Cell Death & Disease

Figure 5

From: Suppression of polyglutamine protein toxicity by co-expression of a heat-shock protein 40 and a heat-shock protein 110

Figure 5

Neuron degeneration in GMR-HQ flies is suppressed by co-expression of DNAJ-1 and HSC70cb. (a) Example of eye morphology of GMR-HQ/ninaE>DNAJ-1 retina from 1-, 5-, 10- and 15-day-old animals by the optical neutralization technique. (b) Time course of photoreceptor degeneration was determined by the optical neutralization assay as showed in a. The flies were reared under a 12 h light/12 h dark cycle at 25 °C. Each data point was based on examination of ≥80 ommatidia from ≥5 flies. Error bars represent the SDs. (c and d) Retinal morphology was examined by TEM. (c) 10-day-old flies of GMR-HQ (GMR-HQ/+), GMR-HQ/ninaE>hsc70cb (GMR-HQ ninaE-Gal4/UAS-hsc70cb), GMR-HQ/ninaE>dnaJ-1(GMR-HQ ninaE-Gal4/UAS-dnaJ-1), and GMR-HQ/ninaE>dnaJ-1 hsc70cb (GMR-HQ ninaE-Gal4/UAS-dnaJ-1 UAS-hsc70cb) were examined. (d) Retina from 15-day-old flies of wild type, GMR-HQ/ninaE>dnaJ-1, and GMR-HQ/ninaE>dnaJ-1 hsc70cb was used. The scale bar in the left panel of c represents 2 μm

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