Figure 1 | Cell Death & Disease

Figure 1

From: MiR203 mediates subversion of stem cell properties during mammary epithelial differentiation via repression of ΔNP63α and promotes mesenchymal-to-epithelial transition

Figure 1

Expression of miR203 correlates with differentiation in the mammary epithelium. (a) Fluorescence-activated cell sorting (FACS) of negative lineage selected mammary epithelial cells excised from the mouse mammary gland and labeled with CD29, CD24 and CD61. Labeled mammary epithelial cells were gated for isolation of MaSCs, luminal progenitor and differentiated luminal cell fractions based on CD29, CD24 and CD61 status. (b) qRT-PCR analysis of isolated mammary cells fractions for detection of mature miR203 expression. (c) Total RNA was isolated from mammary cell fractions for qRT-PCR analysis on ZEB mRNA expression. (d) Immunofluorescent staining against P63 from mammary epithelial stem cells (left) and luminal mammary cells (right) applied to glass microscope slides. (e) qRT-PCR analysis of isolated mammary cell fractions for detection of HBP1 mRNA (transcriptionally repressed by ΔNP63α). (f) Mature miR-203 expression levels normalized to SnU6 from RNA lysates of 10 mammary and breast cancer cell lines using two-step TaqMan assays. All data are means±S.E.; n=2, P-values are indicated

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