Figure 5 | Cell Death & Disease

Figure 5

From: MiR203 mediates subversion of stem cell properties during mammary epithelial differentiation via repression of ΔNP63α and promotes mesenchymal-to-epithelial transition

Figure 5

MiR203 is negatively regulated by ZEB1 and is inversely expressed in mammary and breast cancer cell lines. (a) Total RNA was isolated from five mammary and breast cancer cell lines and qRT-PCR analysis to detect ZEB1 mRNA and mature miR203 was performed. (b and c) IMEC cells were transiently transfected with siRNA against ZEB1 or scramble control (60 nm) and harvested 48 h later for qRT-PCR analysis on mature miR203 (b) and western blot analysis using an anti-P63 antibody (c). (d and e) MCF10A cells were transiently transfected with an empty mammalian expression plasmid (pCDNA-CTR) or one encoding murine Zeb1 (pCDNA-Zeb1). Cells were collected 72 h after transfection and analyzed using qRT-PCR for mature miR203 detection and western blot using anti-Zeb1 and anti-P63 antibodies. Data are means±S.D.; n=3

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