Figure 5
Role of NOX in MA- and/or gp120-induced oxidative stress and cell death. (a and b) SVGA cells were treated with DPI, a selective inhibitor for NOX, at various concentrations 1 h before the treatment with MA and/or gp120 and the effect was observed on ROS production (a) and cell death (b). (c and d) Astrocytes were transfected with 10 nM small interfering RNA (siRNA) targeting either NOX2 or NOX4 subunit of NOX family for 48 h. These cells were further treated with MA and/or gp120. The effect of gene knockdown on ROS production was measured for NOX2 (c) and NOX4 (d). The gene knockdown was confirmed using western blotting for the efficiency of each siRNA. The cells transfected with 10 nM of control siRNA was used as control, which showed no significant change in ROS production. The bars represent mean±S.E. of at least three independent experiments with each treatment in triplicates (except DPI 5 nM in (a) and DPI 25 nM in (b)). The P-value ≤0.05 (*) and ≤0.01 (**) were considered statistically significant using two-tailed Student’s t-test and multiple analysis of variance (ANOVA)
