Figure 6
The expression of ferritin and the role of FWH chemistry in MA- and/or gp120-mediated oxidative stress and cell death. SVGA astrocytes were treated with MA and/or gp120 and the expressions of ferritin HC were measured using western blotting (a) and representative image is shown. Cells were treated with varying concentrations of DFO, an iron chelator used as FWH reaction antagonist, for 1 h before the treatment with MA and/or gp120 and the effect was observed on ROS (b) and cell death (c). (d) The amount of carbonylated proteins after 24 h as a result of treatment with MA and/or gp120. The ROS production and cell death were compared with untreated control that was normalized at 100%. The bars represent mean±S.E. of at least three independent experiments with each treatment in triplicates. The P-value ≤0.05 (*) and ≤0.01 (**) were considered statistically significant using two-tailed Student’s t-test and multiple analysis of variance (ANOVA)
