Figure 5 | Cell Death & Disease

Figure 5

From: Phosphorylation of signal transducer and activator of transcription 1 reduces bortezomib-mediated apoptosis in cancer cells

Figure 5

Synergistic cytotoxic effects of the combination of cisplatin (CDDP) and bortezomib (BTZ) in ovarian cancer cells. (a) In the MTT assays, cancer cells were treated with CDDP at different concentrations for 24 h. The EC50 of CDDP was approximately 50 μM. (b) TOV112D cells were treated with 5 μM of CDDP and 0.05 μM of BTZ for 24 h. Cell viability was assessed using the MTT assay. The cytotoxic effects caused by the combined treatment (5 μM of CDDP and 0.05 μM of BTZ) were analyzed using (c) the Cell Death ELISA and (d) the LDH assays. (e) TOV112D cells were exposed to the combination of CDDP (5 μM) and BTZ (0.05 μM) for 24 h, and subsequently analyzed by immunoblotting for the levels of caspase-3 cleavage and STAT1 phosphorylation. For clarity of presentation, data were normalized with that of BTZ-treated cells, which was set as 1 (black bar). Data are expressed as mean±standard error of the mean. The results are representative of at least three independent experiments

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