Figure 1 | Cell Death & Disease

Figure 1

From: Lysyl oxidase activity regulates oncogenic stress response and tumorigenesis

Figure 1

HEC-TM cells enter senescence after MEK activation. (a) HEC-TM cells were treated for 3 days with 4-OHT to activate MEK and were monitored the following days. (b) Cell extracts were prepared and analyzed by immunoblotting with the indicated antibodies at d0 or at d5. Tubulin was used as a loading control. (c) Cells were PFA-fixed and crystal violet-stained. (d) Cells were fixed and assayed for SA-β-Gal activity. (e) RNAs were prepared and RT-qPCRs were performed against the indicated genes. Expression was normalized against the level of actin mRNA. The experiments shown in this figure are representative of at least 3 independent experiments

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