Figure 5
From: Lysyl oxidase activity regulates oncogenic stress response and tumorigenesis
LOX activity favors escape from senescence in vivo in a model of PDAC. Pdx1-Cre;LSL-KrasG12D/+; Ink4a/Arflox/lox (KIA) mice were injected with concentrated LOX or control supernatant every day from day 23 after birth. Mice were killed 39 days after birth and their pancreases fixed before analysis. (a) A Sirius red staining of collagen was performed at the indicated times. Collagen fiber organization is illustrated by pictures made by polarized light microscopy (scale bar: 200 μm). (b) SA-β-Gal assays were performed on pancreas samples at the indicated times. (c) IHC performed against the Dec1 senescence marker. (d) IHC against the Wnt16B senescence marker. (e) IHC against the Ki67 proliferation marker. For (c–e) Scale bar: 50 μm
