Figure 3

MT2A overexpression modulates osteosarcoma cell viability through zinc chelation. SaOS2 and U2OS parental cells were incubated with increasing doses of Zn chelator TPEN. (a) Cell viability was evaluated using the MTT test. (b) SaOS2 and U2OS parental and MT2A-overexpressing cells were incubated with increasing doses of ZnCl₂. Free intracellular zinc content was evaluated by fluorimetry. (c) SaOS2 and U2OS MT2A-overexpressing cells were incubated with increasing doses of ZnCl₂. Parental cells were maintained in normal medium. Cell replication was evaluated using a BrdU incorporation assay. (d) SaOS2 and U2OS parental cells were incubated in chelex-treated medium supplemented with increasing doses of ZnCl₂. Cell replication was evaluated using a BrdU incorporation assay. Results are expressed as mean±S.D. *P<0.05 versus untreated cells