Figure 5 | Cell Death & Disease

Figure 5

From: Zinc chelation: a metallothionein 2A’s mechanism of action involved in osteosarcoma cell death and chemotherapy resistance

Figure 5

MT2A modulates p53 conformation-dependent activity. (a) Relative content of active form of P53 was evaluated by immunoprecipitation using a conformational-dependent antibody. (b) P21 protein levels were evaluated in U2OS parental and MT2A-overexpressing cells by western blot analysis. (c,d) P73 and P63 protein levels were evaluated in U2OS parental and MT2A-overexpressing cells by western blot analysis. (e) MT2A protein levels were evaluated in U2OS cells incubated in the presence or absence of doxorubicin (100 ng/ml). (f) Relative content of active form of P53 was evaluated by immunoprecipitation using a conformational-dependent antibody

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