Table 1 Transcripts expression level in human (SaOS2) osteosarcoma cells

From: Zinc chelation: a metallothionein 2A’s mechanism of action involved in osteosarcoma cell death and chemotherapy resistance

 

Fold change after 6 h

Fold change after 15 h

Fold change after 24 h

Metallothioneins family

 MT1A

0.97 (P>0.10)

0.96 (P>0.10)

1.10 (P>0.10)

 MT1B

0.97 (P>0.10)

0.99 (P>0.10)

1.48 (P>0.10)

 MT1E

0.97 (P>0.10)

1.04 (P>0.10)

0.89 (P>0.10)

 MT1F

0.97 (P>0.10)

1.00 (P>0.10)

1.14 (P>0.10)

 MT1G

0.97 (P>0.10)

1.28 (P>0.10)

1.42 (P>0.10)

 MT1J

0.97 (P>0.10)

0.99 (P>0.10)

1.04 (P>0.10)

 MT1K

0.97 (P>0.10)

1.00 (P>0.10)

1.05 (P>0.10)

 MT1L

0.97 (P>0.10)

1.00 (P>0.10)

1.07 (P>0.10)

 MT2A

3.25 (P=0.03)**

2.90 (P=0.05)**

2.85 (P=0.02)**

 MT4

0.97 (P>0.10)

0.92 (P>0.10)

0.94 (P>0.10)

ZnT family

 SLC30A1

1.07 (P>0.10)

1.07 (P>0.10)

1.02 (P>0.10)

 SLC30A2

1.02 (P>0.10)

1.03 (P>0.10)

1.03 (P>0.10)

 SLC30A3

0.93 (P>0.10)

0.98 (P>0.10)

0.93 (P>0.10)

 SLC30A4

0.99 (P>0.10)

1.08 (P>0.10)

1.01 (P>0.10)

 SLC30A5

0.95 (P>0.10)

0.98 (P>0.10)

0.97 (P>0.10)

 SLC30A6

0.97 (P>0.10)

1.20 (P>0.10)

1.05 (P>0.10)

 SLC30A7

0.97 (P>0.10)

1.01 (P>0.10)

0.91 (P>0.10)

 SLC30A8

0.93 (P>0.10)

1.07 (P>0.10)

0.94 (P>0.10)

 SLC30A9

1.07 (P>0.10)

0.96 (P>0.10)

1.05 (P>0.10)

 SLC30A10

1.11 (P>0.10)

0.92 (P>0.10)

1.02 (P>0.10)

ZIP family

 SLC39A1

0.97 (P>0.10)

1.07 (P>0.10)

0.77 (P>0.50)

 SLC39A2

1.01 (P>0.10)

1.07 (P>0.10)

1.06 (P>0.10)

 SLC39A3

1.00 (P>0.10)

0.89 (P>0.10)

0.93 (P>0.10)

 SLC39A4

0.97 (P>0.10)

1.08 (P>0.10)

0.97 (P>0.10)

 SLC39A5

1.01 (P>0.10)

1.01 (P>0.10)

1.02 (P>0.10)

 SLC39A6

1.23 (P>0.10)

1.05 (P>0.10)

0.86 (P>0.10)

 SLC39A7

0.81 (P>0.10)

0.93 (P>0.10)

1.24 (P>0.10)

 SLC39A8

1.01 (P>0.10)

0.99 (P>0.10)

0.88 (P>0.10)

 SLC39A9

1.03 (P>0.10)

1.30 (P>0.10)

0.92 (P>0.10)

 SLC39A10

0.93 (P>0.10)

0.90 (P>0.10)

0.98 (P>0.10)

 SLC39A11

0.97 (P>0.10)

1.07 (P>0.10)

1.18 (P>0.10)

 SLC39A12

1.05 (P>0.10)

0.94 (P>0.10)

1.00 (P>0.10)

 SLC39A14

0.98 (P>0.10)

1.09 (P>0.10)

1.16 (P>0.10)

  1. ZIP, Zrt- and Irt-like protein; ZnT, zinc transporter.
  2. Cells were incubated for 6, 15 or 24 h with/without 10 μM atorvastatin. The mRNA expression level of the main families of molecules involved in zinc homeostasis was evaluated by quantitative RT-PCR. Normalized intensities of each transcript were determined for two independent experiments. Cutoffs for selection are equal to 1 for the absolute value of log2 (ratio), and to 0.05 for the P-value. Fold change of the ratio of atorvastatin/control is indicated. ** indicates P-value<0.05 versus untreated cells.
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