Figure 5
From: Implication of transcriptional repression in compound C-induced apoptosis in cancer cells

The downregulation of Bcl-2 and Bcl-xl promotes compound C-induced apoptosis. (a) A549, SMMC-7721 and HeLa cells were treated with 10 μmol/l compound C for indicated periods and cell lysates were analyzed for the levels of indicated proteins. (b) A549, SMMC-7721 and HeLa cells were treated with 5 μmol/l compound C for 24 h with or without ABT-263 (1 μmol/l) pretreatment for 1 h, and apoptosis were analyzed using flow cytometry after staining with FITC-conjugated Annexin V and propidium iodide. (c) A549 and SMMC-7721 cells were treated with 10 μg/ml ActD or 50 μmol/l DRB for 24 h and cell lysates were analyzed for the levels of indicated proteins. Data are presented as mean values±S.D. of three measurements. Columns, mean of three individual experiments; bars, S.D. * Significantly different from control value; ** Significantly different from *value