Figure 6 | Cell Death & Disease

Figure 6

From: Implication of transcriptional repression in compound C-induced apoptosis in cancer cells

Figure 6

The phosphorylation of eIF2α promotes compound C-induced apoptosis. (a) A549, SMMC-7721 and HeLa cells were treated with indicated amounts of compound C for 24 h and cell lysates were analyzed for the levels of indicated proteins. (b) A549, SMMC-7721 and HeLa cells were treated with 10 μmol/l compound C for indicated periods and cell lysates were analyzed for the levels of indicated proteins. (c) A549, SMMC-7721 and HeLa cells were treated with 10 μg/ml ActD or 50 μmol/l DRB for 12 h and cell lysates were analyzed for the levels of indicated proteins. (d) A549, SMMC-7721 and HeLa cells were treated with 10 μmol/l compound C for 20 h with or without salubrinal (25 μmol/l) pretreatment for 1 h, and apoptosis were analyzed using flow cytometry after staining with FITC-conjugated Annexin V and propidium iodide. (e) After eIF2αS51A expression plasmids transient transfection for 24 h, A549, SMMC-7721 and HeLa cells were treated with 10 μmol/l compound C for 24 h, and apoptosis were analyzed using flow cytometry after staining with FITC-conjugated Annexin V and propidium iodide. Data are presented as mean values±S.D. of three measurements. Columns, mean of three individual experiments; bars, S.D. * Significantly different from control value; ** Significantly different from *value

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