Figure 1 | Cell Death & Disease

Figure 1

From: Androgen receptor-mediated apoptosis in bovine testicular induced pluripotent stem cells in response to phthalate esters

Figure 1

Generation of iPSCs from bovine testicular cells. (a) Typical morphology of bovine iPSC colonies generated using OCT4 on day 25 after electroporation ( × 100 magnification; upper left panel). Alkaline phosphatase staining of bovine iPSCs (lower left panel), and immunocytochemical analysis of pluripotency and surface markers (OCT4, NANOG, SOX2, SSEA-1, and SSEA-4 indicated in green) in bovine iPSCs. Nuclei were stained with 4′,6-diamidino-2-phenylindole (indicated in blue) ( × 200 magnification). (b) Bovine iPSC gene expression. RT-PCR analysis of the transcripts of ‘stemness’ genes (OCT4, SOX2, MYC, KLF4, STAT3, SUZ12, DNMT1, and MEF2A) in bovine testis cells and iPSCs. The primers used for RT-PCR are listed in Table 1. (c) G-banding karyotype analysis of the bovine iPSC cell line. Bovine iPSCs had the normal distribution of 60 chromosomes at passage 15, including the XY sex chromosomes

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