Table 1 Nucleotide sequences of the primers used for stemness-related genes and the expected sizes of the DNA amplicons
|  | Gene | 5′→3′ | Size of amplified DNA (bp) |
|---|---|---|---|
1 | OCT3/4-F | CCCTGAGGAGTCCCAGGACAT | 356 |
| Â | OCT3/4-R | GCAGGAACATGCTCTCCAGGTT | Â |
2 | SOX2-F | CTACAGCATGATGCAGGACCAGCT | 381 |
| Â | SOX2-R | TGCTGGGACATGTGAAGTCTGCTG | Â |
3 | GKLF4-F | GTTCGTGTTGAAGGCGTCGCTG | 173 |
| Â | GKLF4-R | TGCACGAGGAGACAGCCTCCT | Â |
4 | c-MYC-F | CCAAGCTCGTCTCGGAGAAGC | 334 |
| Â | c-MYC-R | TCAGAGTCGCTACTGGTCGTGG | Â |
5 | SALL4-F | CATAGACAAGGCCACCACCGACC | 276 |
| Â | SALL4-R | ATGTGCATGCGGATGTGCTGCT | Â |
6 | ID1-F | ACGACATGAACGGCTGCTACTC | 142 |
| Â | ID1-R | TGGGATTCCGAGTTGAGCTCCAA | Â |
7 | EED-F | ATAGCAATACAAGCCATCCCCTGC | 223 |
| Â | EED-R | AATATTGCCACCAGAGTGTCCGTC | Â |
8 | SUZ12-F | GCAGTTCACTCTTCGTTGGACAGG | 449 |
| Â | SUZ12-R | CCTGAGGATTTCCTGCATAGGAGC | Â |
9 | STAT3-F | GTCTAACAATGGCAGCCTCTCAGC | 405 |
| Â | STAT3-R | AAGAGTTTCTCCGCCAGCGTC | Â |
10 | GADD45A-F | CTTTGGAGGAATTCTCGGCTGGAG | 252 |
| Â | GADD45A-R | CATTCTCACAGCAGAATGCCTGG | Â |
11 | SMAD4-F | TTCATGACTTTGAGGGACAGCCA | 438 |
| Â | SMAD4-R | GCTCATTGTGAACTGGTGGCCAG | Â |
12 | DNMT1-F | CGGTGTTCACAAAGGACTGCAACG | 359 |
| Â | DNMT1-R | GTACTGACCAGCCTGCAGCAC | Â |
13 | DNMT3A-F | TGCAAGAACTGCTTCCTGGAATGC | 398 |
| Â | DNMT3A-R | ACCAGAAGCCCTGTAGCAATTCC | Â |
14 | TERT-F | CCTACGTGGTGGAGCTGCTCAG | 155 |
| Â | TERT-R | TGACAGTTCTCGAAGCCGCAC | Â |
15 | MEF2A-F | ATGCCTCCACTGAATACCCAAAGG | 217 |
| Â | MEF2A-R | ACACCTGTCCCAGAGACAGCAT | Â |
16 | MEF2C-F | GGTATGGCAATCCCCGAAACTCAC | 408 |
| Â | MEF2C-R | GCCAGCCAGTTACTGACCCAAGAT | Â |
