Figure 7
From: Light-controlled inhibition of malignant glioma by opsin gene transfer
Light stimulation inhibited the proliferation and induced the apoptosis of human glioma cells in vivo. (a) Immunostaining of nestin in the control, light, eYFP+light, ChETA, and ChETA+light groups. (b) EdU-positive cells (green) were observed in the control, light, eYFP+light, ChETA, and ChETA+light groups. Hoechst 33342-stained nuclei are shown in blue. (c) Immunostaining of cyclin D in the control, light, eYFP+light, ChETA, and ChETA+light groups. (d) TUNEL staining of apoptotic U87 cells in the control, light, eYFP+light, ChETA, and ChETA+light groups; Hoechst 33342-stained nuclei are shown in blue. (e) Quantification of the TUNEL-positive cells in the control, light, eYFP+light, ChETA, and ChETA+light groups. (f) Schematic drawing for the mechanism of the light-induced regulation of glioma cell proliferation and apoptosis. Bar=50 μm (a–d). ***P<0.001. The experiments were repeated for three times with similar results
