Figure 1

IDS deficit does not critically affect NSC self-renewal. (a) Histogram showing the IDS enzymatic activity in wt and IDS-ko NSCs. Although detectable in wt single stem cells, neurospheres and differentiated, no enzymatic activity could be revealed in IDS-ko cells. (b) Phase-contrast image of a free floating neurospheres culture. Scale bar: 100 μm. (c) Graph showing the proliferation rate of wt NSCs (red, n=2) and IDS-ko NSCs (blue, n=2) when cultured in the presence of both EGF and FGF2. (d) Histogram showing the number of viable wt and IDS-ko NSCs at 0, 24, 48 and 72 h after dissociation. For each line, 2.5 × 10⁵ cells were plated (n=2). (e) Western blot analysis of Notch expression in wt and IDS-ko cells at 0 and 7 div after differentiation. (f and g) Graphs showing the proliferation rate of wt NSCs (red, n=2 cell lines) and IDS-ko NSCs (blue, n=2 cell lines) when cultured only in the presence of EGF (f) or in the presence of FGF2 (g). All results are presented as the average of the two IDS-ko and of the two wt NSC lines, respectively. Values are means±S.E.M