Figure 4

Neurogenic pattern of IDS-ko mouse brain. (a) Hematoxylin–eosin histochemical staining of wt and IDS-ko mouse brains. Scale bar in top and bottom pictures: 100 μm; scale bar in center pictures (cortex): 50 μm. (b–d) Confocal microscopy images of SVZ, cc, CTX and OB immunostained with antibodies against the neural markers β-tubIII (b), MAP2 (c), Dcx (c) and MBP (d). A disorganization of β-tubIII+, MAP2+ and MBP+ cells is particularly pronounced in cortex (white arrows). Scale bars: 75 μm. (e) Confocal microscopy images of wt and IDS-ko mouse brain immunostained for the astroglial GFAP (e) marker. The morphology of GFAP+ cells is radial glia-like in IDS-ko SVZ (upper right), whereas star-shaped in wt SVZ (upper left). Scale bars: 75–100 μm; in insets: 10–12 μm. (f) Quantification of GFAP+ cells over total DAPI+ nuclei confirms a remarkable astrogliosis in all the brain areas analyzed. Student’s t-test was applied, values are means±S.E.M. cc, corpus callosum; SEPT, septum; CTX, cortex; OB, olfactory bulbs; SVZ, subventricular zone