Figure 3 | Cell Death & Disease

Figure 3

From: Regulation of cell survival by sphingosine-1-phosphate receptor S1P1 via reciprocal ERK-dependent suppression of Bim and PI-3-kinase/protein kinase C-mediated upregulation of Mcl-1

Figure 3

Regulation of pro-apoptotic protein expression in S1P1-expressing CCL39 cells. (a) Control and S1P1-expressing CCL39 cells were switched to serum-free medium (SF) for the indicated times before preparation of detergent-soluble cell extracts. Samples were equalised for protein content before fractionation via SDS-PAGE and subsequent immunoblotting with the indicated antibodies. Quantitation of cleaved caspase-3 and Bim expression normalised to GAPDH is presented as mean values±S.E. for n=3 separate experiments. *P<0.05 versus CCL39 control cells at the indicated time point. (b) Control and S1P1-expressing CCL39 cells were switched to SF medium for the indicated times before preparation of detergent-soluble cell extracts. Samples were equalised for protein content before fractionation via SDS-PAGE and subsequent immunoblotting with the indicated antibodies. Quantitation of Noxa expression normalised to GAPDH is presented as mean values±S.E. for n=3 separate experiments. *P<0.05 versus CCL39 control cells at the indicated time point

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