Figure 6

MiR-296-3p affects the sensitivity of PCa cells to NK cells. (a) NK cells were incubated with anti-CD11α mAbs or isotype-matched IgG and then the cytotoxicity assay was performed against miR-296-3p-overexpressing P69 (P69 miR-296-3p) (left panel) and miR-296-3p-silencing M12 (M12 anti-miR-296-3p) (right panel). (b) The NK cell cytotoxicity (left upper panel), degranulation (right upper panel), perforin polarization (left bottom panel) and conjugate formation (right bottom panel) against miR-296-3p P69 and the ICAM-1-overexpressing miR-296-3p P69 (miR-296-3p+ICAM-1 P69) cells were performed as indicated. (c) The NK cell cytotoxicity (left upper panel), degranulation (right upper panel), perforin polarization (left bottom panel) and conjugate formation (right bottom panel) against the anti-miR-296-3p M12 and the ICAM-1-knockdown anti-miR-296-3p M12 (anti-miR-296-3p+shICAM-1 M12) cells were performed as indicated. (d) The conjugate formation between NK cells and P69 (left panel) or M12 (right panel) stable cell lines after blockade of CD11α on NK cells with anti-CD11α mAbs (isotype-matched IgG as a control) were examined as previously described. All above data were the mean±S.E.M. from three independent experiments