Figure 8

MiR-296-3p-ICAM-1-NK signalling axis affects survival of M12 in peripheral blood. (a) Quantitative analysis of the residual GFP-positive M12 in peripheral blood of nude mice treated with normal rabbit serum. The cells gated on FSC-SSC plot were analyzed (left upper panel) and the number in oval represented percentage of GFP⁺/7-AAD⁻ viable M12 variant cells (left lower panel). The percentage of GFP⁺ M12 variants at three time points of 0, 24 and 36 h was normalized by being divided by percentage of GFP⁺ cells in M12 control cells at 0 h. The data are representative of two independent experiments (n=5) expressed as mean±S.E.M. (right graph). (b and c) Mice were intraperitoneally pre-treated anti-asialo GM1 antibody 1 day before intravenous inoculation of M12 variant cells. Quantitative analysis of residual GFP⁺ M12 variants in peripheral blood of these mice transferred without (b) or with (c) human NK cells at 24 h after intravenous injection of tumour cells. The data were processed and presented as previously described